Kalderon LabColumbia University Biological SciencesColumbia Biology

Microscopy

Confocal image of an ovariole with the germarium at left and progressively larger egg chambers to the right; DAPI labels nuclei blue and Fas3 outlines follicle cells in red.
Multicolor lineage montage with 9-day samples in the top row, 21-day samples in the middle, and 30-day samples at the bottom; later examples contain fewer lineage colors.
Paired germarium images labeled stat and UAS-Hop with Fas3 in red, lineage GFP in green and EdU in blue.
Paired germarium images labeled arr and axn, with green marked cells occupying more posterior positions in arr and extending into the anterior escort-cell region in axn.
A germarium expressing Fly-FUCCI with green-only and overlapping green/red fluorescence among somatic cells.
Four original pupal ovary panels labeled 12, 30, 48 and 102 hours after puparium formation, showing germline and somatic precursor organization.
Wild-type, UAS-Ci-Wt and CRISPR Ci wing discs in three columns, with ptc-lacZ in red above full-length Ci-155 in gray.
Image of a pupal ovary composed of multiple ovarioles with a domain of Wnt signaling shown by the expression of Frizzled3-RFP in red, and precursors to Escort cells and Follicle Stem Cells shown by Traffic Jam staining in green.

Microscopy

Confocal image of an ovariole with the germarium at left and progressively larger egg chambers to the right; DAPI labels nuclei blue and Fas3 outlines follicle cells in red.